Journal: Scientific Reports

Article Title: Barium titanate piezoelectric nanoparticles induce M1 polarization in mouse macrophages via ultrasound in vitro

doi: 10.1038/s41598-025-23364-6

Figure Lengend Snippet: ( A ) Fluorescence images of RAW264.7 cells under different conditions demonstrating bioelectrically-driven differentiation of M0 monocytes into M1 phenotype macrophages. Phase (Column 1), DAPI staining intensity (Column 2), and iNOS staining intensity (Column 3) are shown. Row 1 shows biochemical treatment with IFN-γ + LPS that drives M0 to M1. Row 2 shows positive iNOS intensity effect with pzNP and uS (+ NP, + uS). Rows 3, 4 and 5 represent controls with less/negligible iNOS staining intensity: with pzNPs and without uS (+ NP, –uS, Row 3), without pzNPs and with uS (–NP, + uS, Row 4), and without pzNPs or uS (-NP, -uS, Row 5). Note that the bioelectric combination of pzNP uptake and uS inducing iNOS expression in Row 2 is similar to biochemical treatment with IFN-γ + LPS in Row 1, both indicative of M0→M1 differentiation. ( B ) Average mean iNOS fluorescence intensity of cells within regions of interest from sets of n = 3 fluorescent images quantified using FIJI/ImageJ, for each NP/uS condition shown, plus that for IFN-γ + LPS. A p value > 0.1 (indicated as NS , for Not Significant) was determined via a t -test comparing the IFN-γ + LPS and (+ NP, + uS) data, while p < 0.01 (indicated as **) when comparing those with the three control data sets, as determined via ANOVA analysis. See text and Statistical Analysis for details.

Article Snippet: Then, a primary rabbit polyclonal antibody against mouse iNOS (Cell Signaling Technology, Danvers MA, no. 68186) or mouse arginase (Cell Signaling Technology, no. 93668), a marker for M1, was incubated overnight in PBS (1:500 dilution) at 4 °C.

Techniques: Fluorescence, Staining, Expressing, Control

Journal: Scientific Reports

Article Title: Barium titanate piezoelectric nanoparticles induce M1 polarization in mouse macrophages via ultrasound in vitro

doi: 10.1038/s41598-025-23364-6

Figure Lengend Snippet: Composite fluorescence images of a mixture of RAW264.7 macrophage cells exposed and not exposed to piezoelectric nanoparticle (pzNP) uptake, and then subjected to ultrasound (uS) stimulation . The channels displayed include phase contrast (panel A ), DAPI (blue, panel B ) for nuclear staining, iNOS expression (red, panel C ), and SA-FITC coating of pzNPs (green, panel D ), in cells subjected to pzNP uptake with uS stimulation. ( E ) A mixture of nanoparticle and non-nanoparticle loaded cells are detected. The results indicate that discrete subpopulations of cells exposed to pzNPs and uS can be identified. Cells that have taken up pzNPs but do not exhibit iNOS expression (green circle) can be differentiated from subpopulations that have taken up pzNPs and exhibit iNOS expression (white circle). There are subpopulations of cells that were not exposed to pzNPs and when subjected to ultrasound stimulation do not express iNOS expression (yellow circle).

Article Snippet: Then, a primary rabbit polyclonal antibody against mouse iNOS (Cell Signaling Technology, Danvers MA, no. 68186) or mouse arginase (Cell Signaling Technology, no. 93668), a marker for M1, was incubated overnight in PBS (1:500 dilution) at 4 °C.

Techniques: Fluorescence, Staining, Expressing

Journal: Journal of ethnopharmacology

Article Title: Mechanism of Jizhi syrup's prevention and treatment of acute bronchitis based on LPS-iNOS inflammatory mediators' signalling pathway.

doi: 10.1016/j.jep.2024.118708

Figure Lengend Snippet: Figure 7 Results of the effect of JZTJ on LPS induced iNOS protein expression in 710

Article Snippet: 235 USA), horseradish enzyme-labelled goat anti-rabbit IgG (Beijing Zhongshan Jinqiao 236 Biological Co., Ltd.), rabbit anti-mouse iNOS polyclonal antibody (Cell Signaling 237 Technology, USA), rabbit anti-mouse p44/42 MAPK polyclonal antibody (Cell 238 Signaling Technology.

Techniques: Expressing

Journal: Journal of ethnopharmacology

Article Title: Mechanism of Jizhi syrup's prevention and treatment of acute bronchitis based on LPS-iNOS inflammatory mediators' signalling pathway.

doi: 10.1016/j.jep.2024.118708

Figure Lengend Snippet: Figure 9 PCR study of mRNA expression of inflammatory mediators (iNOS, IL-1β, IL-6) in 737

Article Snippet: 235 USA), horseradish enzyme-labelled goat anti-rabbit IgG (Beijing Zhongshan Jinqiao 236 Biological Co., Ltd.), rabbit anti-mouse iNOS polyclonal antibody (Cell Signaling 237 Technology, USA), rabbit anti-mouse p44/42 MAPK polyclonal antibody (Cell 238 Signaling Technology.

Techniques: Expressing

Journal: Journal of ethnopharmacology

Article Title: Mechanism of Jizhi syrup's prevention and treatment of acute bronchitis based on LPS-iNOS inflammatory mediators' signalling pathway.

doi: 10.1016/j.jep.2024.118708

Figure Lengend Snippet: Figure 7 Results of the effect of JZTJ on LPS induced iNOS protein expression

Article Snippet: 235 USA), horseradish enzyme-labelled goat anti-rabbit IgG (Beijing Zhongshan Jinqiao 236 Biological Co., Ltd.), rabbit anti-mouse iNOS polyclonal antibody (Cell Signaling 237 Technology, USA), rabbit anti-mouse p44/42 MAPK polyclonal antibody (Cell 238 Signaling Technology.

Techniques: Expressing

Journal: bioRxiv

Article Title: LDL-Binding IL-10 Reduces Vascular Inflammation in Atherosclerotic Mice

doi: 10.1101/2024.03.04.582839

Figure Lengend Snippet: 2D03-IL-10 reduces immune cell infiltration into the aorta of apoE -/- mice fed a HFD. a) ApoE -/- mice were fed a HFD for 8 weeks (b, c), 5 weeks (d-h), or 2 weeks (i, j). While continuing the HFD, mice then received 4 weekly i.v. injections of 25 µg IL-10 or molar equivalent Fab-IL-10. Number of b) total immune cells (CD45 + ) and c) macrophages (CD11b + F4/80 + ) in the aorta after 12 weeks of HFD by flow cytometry. The C57BL/6 group were aged-matched mice on a normal chow diet. Plots are pooled from n = 3 mice in each of two independent studies. Number of d) total immune cells (CD45 + ), e) macrophages (CD11b + F4/80 + ), f) dendritic cells (CD11c + MHCII hi ), g) T cells (CD3 + ), and h) B cells (CD19 + ) in the aorta after 9 weeks of HFD by flow cytometry. i) Quantification of iNOS + CD68 + cells per total CD68 + cells in the aortic root after 6 weeks of HFD. j) Representative images of iNOS staining in the aortic root. Scale bar = 50 µm. Data points represent individual mice, and lines represent median values (b-i). Statistics performed by one-way ANOVA with Dunnett’s post-test; p values < 0.06 compared to saline are shown.

Article Snippet: Sections were then stained overnight at 4 °C in a humidified chamber with rat monoclonal anti-mouse CD68 (1:400, BioLegend, clone FA-11) and rabbit polyclonal anti-mouse IL-10 (1:25, Bioss, bs-0698R) or rabbit polyclonal anti-mouse iNOS (1:50, Proteintech, 18985-1-AP).

Techniques: Flow Cytometry, Staining, Saline

Journal: Brazilian Journal of Cardiovascular Surgery

Article Title: Vasa Vasorum in Saphenous Vein for CABG: A Review of Morphological Characteristics

doi: 10.21470/1678-9741-2023-0045

Figure Lengend Snippet: Confocal microscopy of 30 µm frozen cross-sections through the adventita of no-touch saphenous vein (~ 30 min to harvesting for coronary artery bypass grafting) co-immunolabelled for inducible nitric oxide synthase (iNOS) and endothelin-1 (ET-1). A) iNOS-immunoreactivity (red) in vasa blood vessels (Bv); B) ET-1-immunoreactivity (bright green) in the same vessels; C) the vessels show patter of endothelial co-localization of iNOS and ET-1 (yellow). Bar: 50 µm. Note the main steps of immunoprocedure involved: (1) fixation with 4% paraformaldehyde; (2) incubation with a rabbit polyclonal antibody to iNOS (Santa Cruz Biotech) and a mouse monoclonal antibody to ET-1 (Peninsula Labs); (3) incubation with a goat anti-rabbit immunoglobulin G Mexa Fluor® 568 (to detect iNOS) and a goat ant i-mouse immunoglobulin G Mexa Fluor® 488 (to detect ET-1) (both from Molecular Probes); (4) embedment in Citifluor; and (5) examination at a laser microscope: Leica DMRBE with SPZ confocal head. The images were collected at 1.5 µm intervals and then merged as maximal projection]. Images are from a Loesch unpublished study. Ct=adventitial connective tissue; nu=endothelial cell nucleus.

Article Snippet: Note the main steps of immunoprocedure involved: (1) fixation with 4% paraformaldehyde; (2) incubation with a rabbit polyclonal antibody to iNOS (Santa Cruz Biotech) and a mouse monoclonal antibody to ET-1 (Peninsula Labs); (3) incubation with a goat anti-rabbit immunoglobulin G Mexa Fluor® 568 (to detect iNOS) and a goat ant i-mouse immunoglobulin G Mexa Fluor® 488 (to detect ET-1) (both from Molecular Probes); (4) embedment in Citifluor; and (5) examination at a laser microscope: Leica DMRBE with SPZ confocal head.

Techniques: Confocal Microscopy, Incubation, Microscopy